Construction and Testing of Solid State Oxygen Sensor Lab Report

Construction and Testing of Solid State Oxygen Sensor - Lab Report Example In the second experiment, the quenching of luminescence is tested. Experimentally, luminescence is developed and quenched. This process is usually undesirable and very high requirement are therefore imposed on the purity of luminescent. Quenching may occur without any permanent change in the molecules, for example with no photochemical reactions. In static quenching, a complex is formed between the fluorophore and the quencher and the complex is nonfloures-cent. Numerous application for quenching is as a result of the requirements of molecular contact, for example quenching measurements can reveal the accessibility of fluorophores to the quencher.Both static and dynamic quenching requires molecular contact between the fluorophore and the quencher. For collisional quenching, the quencher must diffuse to the fluorophore during lifetime of the excited state. When in contact, the fluorophore returns to the ground state without emitting photons. For a static and dynamic quenching to occur , the fluorophore and the quencher must be in contact.In this experiment, tri (2,2†²-bipyridine) ruthenium (II) hexafluorophosphate is used with two quenchers namely phenothiazine (PTZ) and N, N, N’, N’-tetramethyl-p phenylenediamine (TMPD).In the third experiment, chemical electronic transitions that are usually induced through the absorption of visible light spectrum result in excited state molecules. In the process, energy is absorbed. The absorbed energy must be released to return the excited species back to the original ground state.